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KMID : 0613820110210050706
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Journal of Life Science
2011 Volume.21 No. 5 p.706 ~ p.712
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Identification and Purification of New Brassinosteroid-Conjugates in Arabidopsis thaliana
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Joo Se-Hwan
Lee Yew
Kim Seung-Ki
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Abstract
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Metabolism of ³H-castasterone in the presence of ³²P-ATP was examined by an enzyme solution prepared from A. thaliana after a reversed phased HPLC, after which a polar metabolite labeled by both ³H and ³²P was obtained, suggesting that ³H-CS is phosphorylated by ³²P-ATP. To elucidate the structure of the phosphorylated CS, the same enzyme assay was carried out with non-isotopes labeled CS and ATP. In GC-MS analysis the metabolite gave a molecular ion at m/z 664 as a bismethanboronate, suggesting the metabolite is a CS phosphate. Treatment of wheat germ acid phosphatase that hydrolyzed phosphoester bond gave the same mass spectrum and GC retention time in GC-MS analyses, confirming that the metabolite is phosphorylated CS. This is the first example of phosphorylated conjugates of CS in plants.
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KEYWORD
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Brassinosteroids, conjugation, inactivation, phosphorylation
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